Role of Plasma Triglyceride Turnover in Deposition of Newly Synthesized Fatty Acid in Adipose Tissue

Abstract

Rats were injected intravenously or subcutaneously with ³H-H₂O and then intravenously with serum containing ¹⁴C-labeled triglyceride (TG), collected from a donor rat previously injected with palmitate-1-¹⁴C. Recipient animals were studied at various times in relation to feeding, and thus had widely different rates of adipose tissue lipogenesis during the 30-minute observation period before sacrifice. Analyses of the time course of ¹⁴C of plasma TG, of the ³H content of the plasma TG and of liver lipid at sacrifice, and of the ¹⁴C/³H ratio in adipose tissue led to the conclusion that transport through the plasma plays an insignificant role in the deposition of newly synthesized fatty acid in the adipose tissue of the rat.