Mechanism of Protein Chain Termination: Further Characterization of a Mutant Defective in a New Protein Synthesis Factor
- 1 January 1973
- journal article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 70 (1), 31-35
- https://doi.org/10.1073/pnas.70.1.31
Abstract
Mutant N4316 is conditionally lethal at 43 degrees . At 36 degrees it suppresses the termination codons UGA and UAA, but not the UAG codon or a missense mutant of T4 bacteriophage. In vitro, a factor rescues protein synthesis from a temperature-dependent arrest when N4316 extracts are used with RNA from bacteriophage f2. Analyses of the substrate in the arrested synthesis and of the product of the rescue reaction indicate that the factor works at the level of coat protein termination, and that it also affects the synthesis of noncoat protein products. The rescue factor is different from the release factors RF-1, RF-2, and RF-3. Model systems previously used to study release fail to score for at least one vital function in protein chain termination.Keywords
This publication has 28 references indexed in Scilit:
- Mechanism of polypeptide chain termination. Translation of tandem amber termination codons by an amber suppressor transfer ribonucleic acid.1972
- More mutant tyrosine transfer ribonucleic acidsJournal of Molecular Biology, 1970
- Release Factor Translation of RNA Phage Terminator CodonsNature, 1970
- Nucleotide Sequence from the Polypeptide Chain Termination Region of the Coat Protein Cistron in Bacteriophage R17 RNANature, 1970
- Fate of the message-ribosome complex upon translation of termination signalsJournal of Molecular Biology, 1969
- MUTANTS IN Escherichia coli RIBOSOMES: A NEW SELECTIONProceedings of the National Academy of Sciences, 1969
- Peptide Chain Termination, Codon, Protein Factor, and Ribosomal RequirementsCold Spring Harbor Symposia on Quantitative Biology, 1969
- Sequential Translation of Trinucleotide Codons for the Initiation and Termination of Protein SynthesisScience, 1968
- Studies on the mechanism of polypeptide chain termination in cell-free extracts of E. coli.Proceedings of the National Academy of Sciences, 1966
- Polypeptide chain tennination using synthetic polynucleotidesJournal of Molecular Biology, 1965