DNA Synthesis by RNA-Containing Tumor Viruses
- 1 October 1970
- journal article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 67 (2), 1034-1041
- https://doi.org/10.1073/pnas.67.2.1034
Abstract
Murine leukemia (Rauscher and Moloney strains) and sarcoma (Kirsten strain) virions, as well as the mammary tumor virus of mice, contain an RNA-dependent DNA polymerase. Optimal incorporation of deoxyribonucleoside triphosphates occurs at a critical detergent (Triton X-100) concentration (0.010-0.014%). At higher than optimal detergent concentrations the virion is seen to be disrupted and enzyme activity is lost. The virion, enzymatic activity, and newly synthesized DNA all cosediment in a sucrose gradient. Thus far the enzymatic activity has been found only in RNA viruses that have oncogenic properties.Keywords
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