Differentiation of Types 1 and 2 Herpes Simplex Virus by Plaque Inhibition with Sulfated Polyanions

Abstract

The relative inhibition of Type 1 and Type 2 herpes simplex virus plaque formation by natural and synthetic poly–anions has been studied. Type 1 and Type 2 HSV were found to have different sensitivities to several naturally occurring sulfated acid mucopolysaccharides and to the synthetic polyanion dextran sulfate. In all instances where sensitivity to a polyanion was observed, it was greater for Type 2 than for Type 1 HSV. These differences were especially marked for dextran sulfate, heparin and agar inhibitor. Chondroitin sulfate B inhibited Type 2 HSV at high concentration, but did not inhibit plaque formation of Type 1.