Costs were estimated for vegetatively producing plants of broccoli (Brassica oleracea L. (Italica group) through tissue culture with 3 culture stages and growing the plants 7 weeks in the greenhouse to field transplant size. The analysis was based on producing 11,000 finished transplants per week. Unit costs were estimated at 11.6¢ for tissue culture production of each plantlet and an additional 3.8¢ greenhouse cost for growing to transplant size.