Cytoplasmic inheritance of rutamycin resistance in mouse fibroblasts.
- 1 January 1978
- journal article
- research article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 75 (1), 324-328
- https://doi.org/10.1073/pnas.75.1.324
Abstract
Mouse fibroblasts resistant to the drug rutamycin were isolated by selectively introducing BrdUrd [bromodeoxuridine] into the mitochondrial genome of a line of mouse fibroblasts (clone 1 D) lacking a cytoplasmic thymidine kinase enzyme. The ATPase (ATP phosphohydrolase; EC 3.6.1.3) activity of mitochondria isolated from these cells was resistant to rutamycin. The rutamycin-resistant mutants were enucleated with cytochalasin B and fused with mouse A 9 cells resistant to 8-azaguanine and sensitive to rutamycin. Cytoplasmic hybrids, or cybrids, were selected as cells resistant to rutamycin and 8-azaguanine, and appeared at a high frequency. Other fusions between rutamycin-resistant nucleated cells and A 9 produced colonies at a much lower frequency. Fusions between enucleated clone 1 D cells and A 9 cells produced no rutamycin-resistant colonies. Rutamycin resistance is probably a cytoplasmically inherited characteristic in this cell line.Keywords
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