Glucose Transport into Spinach Chloroplasts

Abstract

The uptake of radioactively labeled hexoses and pentoses into the sorbitol-impermeable ³H₂O space (the space surrounded by the inner envelope membrane) of spinach (Spinacia oleracea L.) chloroplasts has been studied using silicone layer filtering centrifugation. Of the compounds tested, d-xylose, d-mannose, l-arabinose, and d-glucose are transported most rapidly, followed by d-fructose and l-arabinose. The rate of l-glucose uptake is only about 5% of that of d-glucose.