Further Investigations of the γ-Chain in a Ghanaian Adult, Homozygous for Hereditary Persistence of Fetal Haemoglobin

Abstract

The γ chain in a Ghanaian homozygous for hereditary persistence of fetal haemoglobin was considered to be of the G_γtype on the basis of the amino acid analysis of _γTp XIV (_γ133–144) of the Hb F of this subject [1]. Recently, the sequence of residues _γ134–137 of the _γ-chain of this subject was determined and found to contain some alanine at position _γ136. It is therefore of the G_γ + A_γtype. A rapid technique for the isolation of _γCB-3 (_γl34–146) peptides in human fetal haemoglobin for G_γ;A_γratio determination is described.