Separation of Specific Initiation Factors Involved in the Translation of Myosin and Myoglobin Messenger RNAs and the Isolation of a New RNA Involved in Translation
- 1 June 1974
- journal article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 71 (6), 2428-2431
- https://doi.org/10.1073/pnas.71.6.2428
Abstract
Two messenger specific factors, phosphocellulose fractions 3 and 4, have been isolated from the initiation factor 3 fraction of red muscle initiation factors by chromatography on phosphocellulose. When added to a reticulocyte cell-free system containing both myoglobin and myosin mRNAs, phosphocellulose fraction 3 is found to specifically stimulate the synthesis of myoglobin while phosphocellulose fraction 4 is found to specifically stimulate the synthesis of myosin. In addition, a new RNA, isolated from the initiation factor 3 fraction, is shown to specifically inhibit the translation of heterologous mRNAs.Keywords
This publication has 13 references indexed in Scilit:
- Translation of myoglobin messenger ribonucleic acidBiochemistry, 1973
- Regulation of protein synthesis in mammalian cells: V. Further studies on the effect of actinomycin D on translation control in Hela cellsJournal of Molecular Biology, 1973
- The effects of inhibitors of RNA and DNA synthesis on protein synthesis and polysome levels in mouse L‐cellsJournal of Cellular Physiology, 1973
- Discrimination between Messenger Ribonucleic Acids by a Mammalian Translation Initiation FactorProceedings of the National Academy of Sciences, 1973
- Purification of a Messenger‐Specific Initiation Factor from Ascites‐Cell SupernatantEuropean Journal of Biochemistry, 1973
- Inhibition of Cellular Protein Synthesis by Double-Stranded RNA: Inactivation of an Initiation FactorProceedings of the National Academy of Sciences, 1973
- Translational Control of β and α Globin Chain SynthesisNature New Biology, 1972
- Myosin synthesis and specificity of eukaryotic initiation factorsBiochemistry, 1972
- Double-Stranded Poliovirus RNA Inhibits Initiation of Protein Synthesis by Reticulocyte LysatesProceedings of the National Academy of Sciences, 1971
- Hemoglobin messenger ribonucleic acid. Distribution of the 9S ribonucleic acid in polysomes of different sizesBiochemistry, 1969