Hydroxyindole‐O‐Methyltransferase in Y‐79 Human Retinoblastoma Cells: Effect of Cell Attachment
- 30 April 1987
- journal article
- research article
- Published by Wiley in Journal of Neurochemistry
- Vol. 48 (5), 1612-1616
- https://doi.org/10.1111/j.1471-4159.1987.tb05709.x
Abstract
Hydroxyindole-O-methyltransferase (HIOMT), the enzyme in the final step of melatonin synthesis, is present in the Y-79 human retinoblastoma cell line. Using electroblot immunolabelings, a single band corresponding to HIOMT was observed. Immunofluorescence, sodium dodecyl sulfate-polyacrylamide gel electrophoresis, and quantification of enzyme activity all revealed dramatic increases in HIOMT in cells attached to substrate compared to cells in suspension culture.Keywords
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