Antibody dependent cell-mediated cytotoxicity againstTrypanosoma cruzi

Abstract

This paper describes in vitro antibody dependent cytotoxicity against Trypanosoma cruzi epimastigotes by normal mouse splenic lymphocytes. Cytotoxicity was expressed as the percentage reduction in the number of motile parasites upon incubation with lymphocytes at 37 C in liver infusion tryptose (LIT) medium confirmed loss of motility as a criterion of cytotoxicity. Incubation of T. cruzi at 37 C. The lag phase was prolonged for T. cruzi which had previously been incubated at 37 °C in the absence of cells.