Application of Selected Ion Monitoring to Determination of Platelet-Activating Factor1

Abstract

The selected ion monitoring (SIM) technique was applied to determination of platelet-activating factor (PAF) or acetyl glyceryl ether phosphorylcholine (AGEPC). Two types of PAF, 1-hexadecyl- and 1-octadecyl-2-acetyl-sn-glyceryl-3-phosphorylcholine (C₁₆₌₀ AGEPC and C₁₈₌₀ AGEPC), were found in human neutrophils on the challenge with ionophore A23187. The contents of C₁₆₌₀ AGEPC in 1×10⁷ neutrophil cells of four volunteers, respectively, were 47, 18, 59, and 73 ng and those of C₁₈₌₀ AGEPC were 22, 4, 19, and 31 ng.