Enhancement of phenolic content, antioxidant and cytotoxic activities of Moringa oleifera leaf and seed by suspension culture
- 25 March 2020
- journal article
- research article
- Published by Informa UK Limited in Natural Product Research
- Vol. 35 (23), 5233-5237
- https://doi.org/10.1080/14786419.2020.1744137
Abstract
Moringa oleifera Lam. family Moringaceae is well known for a wide range of biological activities and a complex phytochemical composition. The current study investigates tissue culture protocols for Moringa oleifera leaves and seeds. For static culture initiation, Murashige and skooge (MS) as a basal medium with hormonal supply of (0–10 µM) of 2,4-dicholorophenoxy acetic acid and 6-benzyl aminopurine for Moringa oleifera seeds and leaves was employed. Suspension cultures with the optimum hormonal combination was initiated for both seeds and leaves calli. Liquid chromatography/mass spectroscopy (LC/MS) analysis performed, for the first time, on the methanolic extracts of plant parts and the produced calli revealed varying concentrations of nine major components (six flavonoids and three phenolic acids). Antioxidant and cytotoxic activities, against three cell lines, were evaluated for the obtained methanolic extracts. In general, superior biological activities were identified for the produced calli when compared to plant parts.Keywords
This publication has 11 references indexed in Scilit:
- Antioxidant Activity of Moringa oleifera ExtractsIndonesian Journal of Chemistry, 2016
- Cultivation, Genetic, Ethnopharmacology, Phytochemistry and Pharmacology of Moringa oleifera Leaves: An OverviewInternational Journal of Molecular Sciences, 2015
- Predicting retention time in hydrophilic interaction liquid chromatography mass spectrometry and its use for peak annotation in metabolomicsMetabolomics, 2014
- Yield improvement strategies for the production of secondary metabolites in plant tissue culture: silymarin from Silybum marianum tissue cultureNatural Product Research, 2014
- Evaluation of cytotoxicity of Moringa oleifera Lam. callus and leaf extracts on Hela cellsAdvanced Biomedical Research, 2014
- Cytotoxic Effects of Bangladeshi Medicinal Plant ExtractsEvidence-Based Complementary and Alternative Medicine, 2011
- Phenolics ofMoringa oleiferaleavesNatural Product Research, 2007
- Moringa oleifera: a food plant with multiple medicinal usesPhytotherapy Research, 2006
- Callus induction and plantlet regeneration in Bixa oreliana L., an annatto-yielding treeIn Vitro Cellular & Developmental Biology - Plant, 2002
- Regulation of plant growth by cytokininProceedings of the National Academy of Sciences, 2001