Lysophosphatidylcholine in oxidized low-density lipoprotein increases endothelial susceptibility to polymorphonuclear leukocyte-induced endothelial dysfunction in porcine coronary arteries. Role of protein kinase C.
- 1 April 1994
- journal article
- abstracts
- Published by Wolters Kluwer Health in Circulation Research
- Vol. 74 (4), 565-575
- https://doi.org/10.1161/01.res.74.4.565
Abstract
We have shown that transferred lysophosphatidylcholine (lysoPC) from oxidized low-density lipoprotein (Ox-LDL) to endothelial surface membrane activates protein kinase C (PKC) in endothelial cells, suggesting that Ox-LDL could alter endothelial functions through PKC activation. The purposes of the present study were to examine whether the endothelial susceptibility to polymorphonuclear leukocytes (PMNs) may be altered in Ox-LDL-treated coronary arteries, which have properties closely resembling those observed in atherosclerotic arteries, and to determine the mechanism(s) by which Ox-LDL may affect the endothelial susceptibility to PMNs. Isolated porcine coronary arteries were cannulated and perfused with oxygenated culture medium with or without LDLs or lipids at a constant flow (37 degrees C, pH 7.4). The treatment of porcine coronary arteries with Ox-LDL increased endothelial adhesiveness to PMNs and augmented PMN-induced impairment of endothelium-dependent arterial relaxation (EDR). Furthermore, Ox-LDL...Keywords
This publication has 25 references indexed in Scilit:
- Activated Human Polymorphonuclear Leukocytes Elicit Endothelium-Dependent Contraction in Isolated Pig Coronary ArteriesJournal of Cardiovascular Pharmacology, 1993
- ICAM-1 (CD54): a counter-receptor for Mac-1 (CD11b/CD18).The Journal of cell biology, 1990
- Rapid neutrophil adhesion to activated endothelium mediated by GMP-140Nature, 1990
- Evidence for the presence of oxidatively modified low density lipoprotein in atherosclerotic lesions of rabbit and man.JCI Insight, 1989
- Modulation of endothelial cell expression of intercellular adhesion molecule 1 by protein kinase c activationBiochemical and Biophysical Research Communications, 1989
- Calphostin C (UCN-1028C), a novel microbial compound, is a highly potent and specific inhibitor of protein kinase CBiochemical and Biophysical Research Communications, 1989
- Impaired muscarinic endothelium-dependent relaxation and cyclic guanosine 5'-monophosphate formation in atherosclerotic human coronary artery and rabbit aorta.JCI Insight, 1987
- Staurosporine, a potent inhibitor of phospholipidCa++dependent protein kinaseBiochemical and Biophysical Research Communications, 1986
- The Pathogenesis of Atherosclerosis — An UpdateNew England Journal of Medicine, 1986
- Isoquinolinesulfonamides, novel and potent inhibitors of cyclic nucleotide-dependent protein kinase and protein kinase CBiochemistry, 1984