Exchange of valine-oxygen during the biosynthesis of δ-(L-α-aminoadipoyl)-L-cysteinyl-D-valine

Abstract
Incubation of [18O2]valine with purified ACV synthetase from Cephalosporium acremonium gave exclusive incorporation of a single 18O label into δ-(L-α-aminoadipoyl)-L-cysteinyl-D-valine (ACV), consistent with the formation of a covalent valinoyl-ACV synthetase complex.