Effect of modulation of protein kinase C on the cAMP‐dependent chloride conductance in T84 cells
- 12 October 1992
- journal article
- Published by Wiley in FEBS Letters
- Vol. 311 (1), 25-28
- https://doi.org/10.1016/0014-5793(92)81358-s
Abstract
The regulation of chloride conductance was investigated in the T84 human colon carcinoma cell line by the quenching of the fluorescent probe 6-methoxy- N -(3-sulfopropyl)quinolinium. The permeable cAMP analog 8-Br-cAMP (100 μ) and the calcium ionophore ionomycin (1 μM) activate a chloride conductance. A prolonged (4 h) preincubation of cells with phorbol 12-myristate 13-acetate (100 nM) or with the diacylglycerol analog 1-oleoyl-2-acetyl-glycerol (100 μM): (1) down-modulates to almost zero the protein kinase C activity in the membranes; (ii) inhibits the activation of the chloride conductance mediated by 8-Br-cAMP but not by calcium; (iii) reduces the mRNA without changing the expression of the protein product of the cystic fibrosis gene. The data suggest that PKC is essential for the activation of the cAMP-dependt chloride conductance in T84 cells.Keywords
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