A rapid and simple method for extracting human immunodeficiency virus type 1 RNA from plasma: enhanced sensitivity
- 1 May 1997
- journal article
- Published by American Society for Microbiology in Journal of Clinical Microbiology
- Vol. 35 (5), 1278-1280
- https://doi.org/10.1128/jcm.35.5.1278-1280.1997
Abstract
We present here a rapid and simple technique for processing human immunodeficiency virus (HIV)-infected plasma by high-speed centrifugation. HIV type 1 virions are pelleted from up to 1 ml of plasma, gently lysed with a nonionic detergent, and directly amplified. The procedure has few manipulations and requires approximately 1.5 h for the processing of 24 samples. Viral recovery ranges from 80 to 90%, with an analytical sensitivity approaching 20 copies/ml.Keywords
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