Isolation and primary structure of the gene encoding fructose-1,6-bisphosphatase fromSaccharomyces cerevisiae
- 15 August 1988
- journal article
- Published by Wiley in FEBS Letters
- Vol. 236 (1), 195-200
- https://doi.org/10.1016/0014-5793(88)80313-2
Abstract
The gene encoding Saccharomyces cerevisiae fructose-1,6-bisphosphatase (FBP1) was isolated. Constructed fbpi::HIS3 null mutants were unable to grow with ethanol, and growth was restored after transformation with the cloned fbp gene. The gene codes for a protein of 347 amino acid residues with an M r of 38131. Homology with the pig kidney cortex and the sheep liver enzyme is 47.7% and 46.6%, respectively, within a central core of 328 amino acid residues. The cloned promoter size was 318 bp and allowed only low level expression of the gene. This indicates a positive activation site (UAS) upstream of the cloned DNA fragment.Keywords
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