Parabrachial nucleus projection towards the hypothalamic supraoptic nucleus: Electrophysiological and anatomical observations in the rat

Abstract

It has been proposed that the pontine parabrachial nucleus (PBN) participates in the regulation of body fluid balance and the release of vasopressin from the neurohypophysis, although the pathways mediating the latter response are uncertain. This study in the rat, utilizing anatomical and electrophysiological methods, describes a projection from the lateral PBN towards the hypothalamic supraoptic nucleus (SON). Rats received iontophoretic injections of the anterograde tracer Phaseolus vulgaris leucoagglutinin (PHA‐L, 2% solution). After 14–17 days, rats were sacrificed and their brains prepared for immunofluorescent visualization of projections to the SON region. PHA‐L‐labelled terminals were found primarily in perinuclear regions immediately dorsal to the SON. In contrast, injections within the medial PBN and the nearby Kölliker‐Fuse nucleus did not reveal labelling in or around the SON. Extracellular recordings from 86 of 118 antidromically identified neurons in anaesthetized rats revealed a set of complex synaptic responses after stimulation in the PBN. Excitatory responses (in 82 of 86 cells) of short (< 100 msec, 39/82 cells) and long (> 100 msec, 43/82) duration were observed in both vasopressin‐ and oxytocin‐secreting cells of the SON, while 4/86 cells displayed a depressant response to PBN stimulation. In the adjacent perinuclear zone, 22/39 nonneurosecretory cells responded with an increase in their excitability consequent to an identical stimulus. These data suggest a predominantly facilitatory influence of lateral PBN neurons on SON neurosecretory cells in the rat, and that the PBN‐SON projection is an indirect one that utilizes an interneuronal network located in the perinuclear zone adjacent to the SON.