Development of a gas chromatographic selected ion monitoring assay for thyroxine (T4) in human serum
- 1 August 1984
- journal article
- research article
- Published by Wiley in Journal of Mass Spectrometry
- Vol. 11 (8), 421-427
- https://doi.org/10.1002/bms.1200110811
Abstract
The components of a gas chromatographic mass spectrometric-selected ion monitoring (SIM) assay for thyroxine (T4) in human serum are described. The internal standard for the assay was synthesized from deuterium-labelled 3,5-diiodotyrosine and 3,5-diiodo-4-hydroxyphenylpyruvic acid. A novel method was developed for isolating the products of the coupling reaction. The results obtained by gas chromatography mass spectrometry SIM were compared with those of radioimmunoassay. The gas chromatographic mass spectrometric SIM assay would form the basis of a reference assay for T4.Keywords
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