Granule contents from rat polymorphonuclear leukocytes: antimicrobial properties and characterization
- 1 June 1986
- journal article
- research article
- Published by Canadian Science Publishing in Canadian Journal of Microbiology
- Vol. 32 (6), 498-504
- https://doi.org/10.1139/m86-091
Abstract
The nonoxidative antibacterial properties of isolated rat polymorphonuclear leukocyte granule contents were examined using Salmonella typhimurium LT-2 and a series of progressively rough lipopolysaccharide mutants of this strain as target bacteria. The granule extract was most active at 37 °C, with a substantial decrease in activity observed at lower temperatures. Deep rough bacterial mutants were killed best within a pH range of 6–8, while killing of mutants with increased lipopolysaccharide content was most efficient at an acid pH of 5. The activity of the extract was dependent on incubation time but was independent of the number of bacterial cells present in the assay mixture. The killing action of the granule extract was inhibited by the cations Na+, K+, Mg2+, Ca, and Fe2+. The degree of inhibition was dependent on the type and concentration of ion used. Rough mutants grown with aeration to log phase were killed more efficiently than the same mutants grown to stationary phase under static conditions. Also, gram-positive bacteria were more susceptible to the extract than were gram-negative organisms.This publication has 25 references indexed in Scilit:
- Oxygen Metabolism and the Toxic Properties of PhagocytesAnnals of Internal Medicine, 1980
- The Extracellular Release of Granulocyte Collagenase and Elastase during Phagocytosis and Inflammatory ProcessesScandinavian Journal of Haematology, 1977
- Susceptibility of Lipopolysaccharide Mutants to the Bactericidal Action of Human Neutrophil Lysosomal FractionsInfection and Immunity, 1977
- Ultrastructural localization of peroxidase in leukocytes of rat bone marrow and bloodThe Anatomical Record, 1977
- Mechanisms for the microbicidal activity of cationic proteins of human granulocytesInfection and Immunity, 1976
- A Rapid and Sensitive Method for the Quantitation of Microgram Quantities of Protein Utilizing the Principle of Protein-Dye BindingAnalytical Biochemistry, 1976
- Neutral proteinases of human spleen. Purification and criteria for homogeneity of elastase and cathepsin GBiochemical Journal, 1976
- ARGININE-RICH PROTEINS OF POLYMORPHONUCLEAR LEUKOCYTE LYSOSOMESThe Journal of Experimental Medicine, 1968
- The measurement of lysozyme activity and the ultra-violet inactivation of lysozymeBiochimica et Biophysica Acta, 1952
- PROTEIN MEASUREMENT WITH THE FOLIN PHENOL REAGENTJournal of Biological Chemistry, 1951