Der katalytische Effekt von Thorium- und Zirkonium-Ionen auf die Hydrolyse von Nucleotiden

Abstract

Ribonucleic acid and deoxyribonucleic acid are dephosphorylated in alkaline medium by thorium ions; zirconium ions have no measurable activity. In alkaline medium, in the presence of thorium ions, ribonucleic acid is dephosphorylated more rapidly than deoxyribonucleic acid. The 3[image]-nucleotides of adenosine, guanosine, cytidine and uridine are dephosphoryiated by thorium and zirconium ions, in alkaline and acidic media, much more rapidly than polynucleotides (ribonucleic acid and deoxyribonucleic acid). Thorium ions are more active than zirconium ions. The rates of hydrolysis of mononucleotides by thorium ions in alkaline medium decrease in the order: "3"-AMP, "3"-CMP, "3"-UMP, "3"-GMP; this also applies to an acidic medium after a reaction time of 24 hours.