Characterization of a Feline Leukemia Viral Cell Membrane Antigen Reactive with Feline Sera
- 1 December 1977
- journal article
- research article
- Published by The American Association of Immunologists in The Journal of Immunology
- Vol. 119 (6), 2194-2202
- https://doi.org/10.4049/jimmunol.119.6.2194
Abstract
Cats which have been infected with FeLV and which have recovered without disease or persistent viremia are at a low to negligible risk for subsequent development of FeLV-associated neoplasia. To evaluate the immunologic correlates of this protection, cat sera from various free-living populations were shown by immunoprecipitation of FL74 cell surface proteins to react with an 85,000 dalton cell surface protein, p85. All sera from non-viremic cats with FL74 cell membrane immunofluorescence reactivity greater than 1:32 precipitated p85. The reactivity of p85 and concomitant cell membrane reactivity could be completely removed from serum with freshly purified, undisrupted FeLV and could be blocked with specific antisera to known virion envelope glycoproteins, demonstrating that p85 contains determinants of the FeLV envelope glycoprotein. The reactivity to p85 could also be removed with FeLV-infected feline and nonfeline cells but not with cells nonproductively transformed by the feline sarcoma virus. These data strongly suggest that a major cell membrane antibody in feline sera is directed to the major FeLV envelope glycoprotein expressed on the surface of cells infected with FeLV but not FeSV. The relationship of this reactivity to the previously and presently defined anti-“FOCMA” reactivity is discussed.This publication has 16 references indexed in Scilit:
- Horizontal transmission of feline leukemia virus under natural conditions in a feline leukemia cluster householdInternational Journal of Cancer, 1977
- Properties of mouse leukemia viruses XIII. Serum therapy of virus-induced murine leukemiasVirology, 1976
- Natural Immunity in Cats to Feline Leukemia Viral AntigensThe Journal of Immunology, 1976
- Presence of murine leukemia virus envelope proteins gp70 and p15(E) in a common polyprotein of infected cellsJournal of Virology, 1976
- gag Gene of mammalian type-C RNA tumour virusesNature, 1976
- Subunit structure of the glycoprotein complex of avian tumor virusJournal of Virology, 1976
- Humoral Immune Responses of Cats to Feline Leukemia Virus: Comparison of Responses to the Major Structural Protein p30 and to a Virus-Specific Cell Membrane Antigen (FOCMA)2JNCI Journal of the National Cancer Institute, 1976
- Biosynthesis of Rauscher leukemia viral proteins: Presence of p30 and envelope p15 sequences in precursor polypeptidesVirology, 1976
- Demonstration of Cellular Antigens on Sarcoma Cells by an Indirect125 I-Labeled Antibody Technique2JNCI Journal of the National Cancer Institute, 1968
- PROTEIN MEASUREMENT WITH THE FOLIN PHENOL REAGENTJournal of Biological Chemistry, 1951