Effects of dolichol and dolichyl phosphate on in vitm differentiation of hematopoietic progenitors

Abstract

The exogenous addition of dolichyl phosphate (Dol-P), an active form of dolichol pol) that carries oligosaccharide chains for protein-N-glycosylation, significantly enhanced colony formation of mouse bone marmw hematopietic pmgenitors (CFUe, BFUe, and CFU-gm) was stimulated by erythropietin (Epo) and colony-stimulating factor (CSF), but Do1 enhanced colony formation of CFUe only. The effects of Do1 or Dol-P on these hematopoietic progenitors were fully dependent on stimulation by Epo or CSF. Other mevalonate-metabolites, such as cholesterol, coenzyme Q¹⁰, and isopentenyladenine, had no effect on hematopoietic progenitors. These studies suggest that exogenous Dol-P enhances the frequency of differentiation of hematopoietic progenitors stimulated by Epo or CSF, and there may be a diversity in cellular response of these progenitors to Dol.