Characterization of a CD11c-Reactive Monoclonal Antibody (HC1/1) Obtained by Immunizing with Phorbo1 Ester Differentiated U937 Cells
- 1 April 1988
- journal article
- research article
- Published by Mary Ann Liebert Inc in Hybridoma
- Vol. 7 (2), 167-176
- https://doi.org/10.1089/hyb.1988.7.167
Abstract
A mouse monoclonal antibody (HC1/1) specific for a differentiation marker of human monocytes and granulocytes has been generated by using as immunogen the monocytic cell line U937 differentiated with phorbol esters. This differentiation antigen has been characterized as the p150/95 member of the CD11 family of glycoproteins by cellular distribution studies, immunoprecipitation and competition experiments with MAb 3.9 specific for the CD11c antigen. Immuno-alkaline phosphatase staining of normal tissue sections with the HC1/1 MAb demonstrated that the CD11c antigen is a useful macrophage marker. Furthermore, the MAb HC1/1 stains specifically populations of macrophages on skin and lymph node sections from different pathologies such as Sarcoidosis, Granuloma annulare, Lepromatous leprosy and Toxoplasmosis.This publication has 24 references indexed in Scilit:
- Role of p150,95 in adhesion, migration, chemotaxis and phagocytosis of human monocytesEuropean Journal of Immunology, 1987
- The monoclonal antibodies alpha S-HCL 1 (alpha Leu-14) and alpha S-HCL 3 (alpha Leu-M5) allow the diagnosis of hairy cell leukemiaBlood, 1985
- A human leukocyte differentiation antigen family with distinct alpha-subunits and a common beta-subunit: the lymphocyte function-associated antigen (LFA-1), the C3bi complement receptor (OKM1/Mac-1), and the p150,95 molecule.The Journal of Experimental Medicine, 1983
- Specific antimononuclear phagocyte monoclonal antibodies. Application to the purification of dendritic cells and the tissue localization of macrophages.The Journal of Experimental Medicine, 1983
- Current View on the Mononuclear Phagocyte SystemImmunobiology, 1982
- The Origin, Morphology, and Function of Epithelioid CellsImmunobiology, 1982
- Enhanced autoradiographic detection of 32P and 125I using intensifying screens and hypersensitized filmFEBS Letters, 1977
- Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4Nature, 1970
- THF EARLY STAGES OF ABSORPTION OF INJECTED HORSERADISH PEROXIDASE IN THE PROXIMAL TUBULES OF MOUSE KIDNEY: ULTRASTRUCTURAL CYTOCHEMISTRY BY A NEW TECHNIQUEJournal of Histochemistry & Cytochemistry, 1966
- Ultra-Rapid Fluorescent Labelling of ProteinsNature, 1962