Strong sequence conservation of African swine fever virus p72 protein provides the molecular basis for its antigenic stability
- 1 September 1996
- journal article
- research article
- Published by Springer Nature in Archiv für die gesamte Virusforschung
- Vol. 141 (9), 1795-1802
- https://doi.org/10.1007/bf01718302
Abstract
The major capsid protein p72 of African swine fever virus (ASFV) has long been considered an important immunodominant antigen for serologic diagnosis. Here we describe the cloning and sequence analysis of two p72-coding genes from ASFV strains Uganda (UGA) and Dominican Republic-2 (DR2). Sequence comparison of these genes, together with those from two other ASFV strains (BA71V and E70), demonstrated that the p72 proteins are highly conserved (97.8% to 100% amino acid sequence identity) in strains isolated from different parts of the world. These results support previous observations indicating that p72 is antigenically stable, and provide a useful molecular basis for further development of ASFV serologic tests using this important antigenic molecule.Keywords
This publication has 24 references indexed in Scilit:
- Highly specific confirmatory Western blot test for African swine fever virus antibody detection using the recombinant virus protein p54Journal of Virological Methods, 1995
- Virulent African Swine Fever Virus Isolates Are Neutralized by Swine Immune Serum and by Monoclonal Antibodies Recognizing a 72-kDa Viral ProteinVirology, 1993
- High-level expression in Escherichia coli of the gene coding for the major structural protein (p72) of African swine fever virusGene, 1993
- Expression in vivo and in vitro of the major structural protein (VP 73) of African swine fever virusArchiv für die gesamte Virusforschung, 1992
- Detection of DNA viruses by radioactive and non radioactive DNA probes: Application to African Swine Fever virusArchiv für die gesamte Virusforschung, 1987
- African Swine Fever VirusPublished by Springer Nature ,1985
- African Swine Fever Virus DNA: Restriction Endonuclease Cleavage Patterns of Wild-type, Vero Cell-adapted and Plaque-purified VirusJournal of General Virology, 1982
- A reliable enzyme linked immunosorbent assay for African swine fever using the major structural protein as antigenic reagentArchiv für die gesamte Virusforschung, 1981
- Proteins specified by African swine fever virusArchiv für die gesamte Virusforschung, 1980
- Studies to determine neutralizing antibody in sera from animals recovered from african swine fever and laboratory animals inoculated with african virus with adjuvantsArchiv für die gesamte Virusforschung, 1967