Prevention of Mif Activity by Agents Known to Increase Cellular Cyclic Amp

Abstract

Agents known to increase intracellular levels of cyclic 3′5′ adenosine monophosphate (cyclic AMP), in a variety of tissues were examined for their effect on migration inhibitory factor (MIF)-induced inhibition of macrophage migration. β-Adrenergic agents (isoproterenol and epinephrine) and prostaglandins E₁ and E₂ consistently prevent MIF activity on macrophage migration. Aminophylline also prevents MIF activity. These observations suggest a possible role for cyclic AMP in modulating the biologic activity of MIF. The finding that dibutyryl cyclic AMP inhibits MIF activity supports this view.