Detection of antibodies to pneumococcal capsular polysaccharides by enzyme-linked immunosorbent assay

Abstract

An enzyme-linked immunosorbent assay for the detection of IgG, IgM, and IgA antibodies against each of the 14 polysaccharide antigens contained in a contemporary pneumococcal vaccine is described. A mean total antibody fold increase above 2 from prevaccination to 4 wk postvaccination serum samples was found for all antigens in 12 healthy adults and 6 children. Of all single fold increases determined, 55% were > 2 in the adults; the value was 64% in children. Type-specific polysaccharide antibody of the IgG class was predominant in 4 wk postvaccination serum samples. Further studies with assays such as the described may lead to a better understanding of the immune response to pneumococcal polysaccharides in both normal subjects and patients at increased risk of pneumococcal infection.