Induction of ebna precedes the first cellular S-phase after ebv-infection of human lymphocytes
- 15 February 1978
- journal article
- research article
- Published by Wiley in International Journal of Cancer
- Vol. 21 (2), 157-160
- https://doi.org/10.1002/ijc.2910210205
Abstract
Using a combination of immunofluorescence and autoradiography, we studied the appearance of EBNA and DNA synthesis in cord-blood lymphocytes after infection with EBV derived from the B95-8 cell line. EBNA appeared between 12 and 25 h after addition of the virus. DNA synthesis was detected in EBNA-positive cells approximately 20 h after the appearance of EBNA. This shows that EBNA induction precedes the first cellular S-phase and suggests that the cells have not yet entered the cell-division cycle when EBNA appears. Little, if any, of the total DNA synthesis induced at this stage can be attributed to EBV-mediated immunologic stimulation.This publication has 14 references indexed in Scilit:
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