The effects of acidosis and alkalosis on the metabolism of glutamine and glutamate in renal cortex slices
Open Access
- 1 May 1972
- journal article
- Published by American Society for Clinical Investigation in JCI Insight
- Vol. 51 (5), 1251-1263
- https://doi.org/10.1172/jci106920
Abstract
Studies of the metabolism of glutamine and glutamate by renal cortex slices from acidotic, alkalotic, and control rats were performed. 88-95% of the glutamine and 104-115% of the glutamate taken up from the medium could be accounted for by the products found. Acidosis increased glutamine uptake and conversion to ammonia, CO2, glucose, lactate, pyruvate, lipid, and protein. The increase in glutamine conversion to ammonia after acidosis could be completely accounted for by the associated increase in its conversion to glucose, glutamate, lactate, and pyruvate. When glutamate metabolism was examined, acidosis did not affect substrate uptake but did increase its conversion to ammonia, glucose, lactate, CO2, and lipid. The increase in 14CO2 from U-14C-glutamine and U-14C-glutamate found with cortex slices from acidotic animals could be explained by the CO2 production calculated to be associated with the enhanced conversion of these substrates to other products during acidosis. 14CO2 production from 1.2-14C-acetate was found to be significantly increased in alkalosis rather than acidosis. These studies suggest that in the rat, the rate at which glutamine is completely oxidized in the Krebs cycle is not a factor regulating renal ammonia production. A comparison of the effects of acidbase status on glutamine and glutamate metabolism suggests that either glutamine transport or glutamine transaminase activity are significantly increased by acidosis.Keywords
This publication has 35 references indexed in Scilit:
- Renal metabolic response to acid-base changesJCI Insight, 1970
- Relation of renal gluconeogenesis to ammonia production in the dogAmerican Journal of Physiology-Legacy Content, 1970
- Effect of acid-base status on renal and hepatic gluconeogenesis in diabetes and fastingAmerican Journal of Physiology-Legacy Content, 1969
- Renal metabolic response to acid base changesJCI Insight, 1969
- Thin-layer chromatography of citric acid cycle intermediates, pyruvate and lactateJournal of Chromatography A, 1968
- Pathways of Ammonia Metabolism in the Intact Functioning Kidney of the Dog*JCI Insight, 1967
- Acid-Base Alterations and Renal Gluconeogenesis: Effect of pH, Bicarbonate Concentration, and PCO2*JCI Insight, 1967
- Microdetermination of glutamate in single cat spinal rootsLife Sciences, 1965
- AMINO ACID EXTRACTION AND AMMONIA METABOLISM BY THE HUMAN KIDNEY DURING THE PROLONGED ADMINISTRATION OF AMMONIUM CHLORIDE*JCI Insight, 1963
- High respiratory quotient of dog kidney in vivoAmerican Journal of Physiology-Legacy Content, 1960