Control of a futile urea cycle by arginine feedback inhibition of ornithine carbamoyltransferase in Agrobacterium tumefaciens and Rhizobia
Open Access
- 1 September 1986
- journal article
- research article
- Published by Wiley in European Journal of Biochemistry
- Vol. 159 (3), 507-511
- https://doi.org/10.1111/j.1432-1033.1986.tb09915.x
Abstract
In Agrobacterium tumefaciens and Rhizobia arginine can be used as the sole nitrogenous nutrient via degradation by an inducible arginase. These microorganisms were found to exhibit arginine inhibition of ornithine carbamoyltransferase activity. This inhibition is competitive with respect to ornithine (Km for ornithine = 0.8 mM; Ki for arginine = 0.05 mM). This type of urea cycle regulation has not been observed among other microorganisms which degrade arginine via an arginase. The competitive pattern of this inhibition leads to its being inoperative in ornithine-grown cells, where the intracellular concentration of ornithine is high. In arginine-grown cells, however, the intracellular arginine and ornithine concentrations are compatible with inhibition and ornithine recycling appears to be effectively blocked in vivo.This publication has 39 references indexed in Scilit:
- Substrate specificity and protonation state of ornithine transcarbamoylase as determined by pH studiesBiochemistry, 1985
- Affinity of arginase for ornithine carbamoyltransferase in Saccharomyces cerevisiaeJournal of Molecular Biology, 1976
- Characterization of amino acid pools in the vacuolar compartment of Saccharomyces cerevisiaeArchiv für Mikrobiologie, 1974
- Ammonia inhibition of the general amino acid permease and its suppression in NADPH-specific glutamate dehydrogenaseless mutants of Saccharomyces cerevisiaeBiochemical and Biophysical Research Communications, 1972
- Influence of Environment on the Content and Composition of Microbial Free Amino Acid PoolsJournal of General Microbiology, 1970
- The control of ornithinetranscarbamylase activity by arginase in Saccharomyces cerevisiaeFEBS Letters, 1969
- The Crabtree Effect: A Regulatory System in YeastJournal of General Microbiology, 1966
- Indication of a specific regulatory binding protein for ornithinetranscarbamylase in saccharomyces cerevisiaeBiochemical and Biophysical Research Communications, 1965
- Magnesium-limited growth of Aerobacter aerogenes in a chemostatJournal of General Microbiology, 1965
- Pathway of arginine biosynthesis in yeastBiochemical and Biophysical Research Communications, 1962