Use of high-pressure liquid chromatography for quantitative structure-activity relationship studies of sulfonamides and barbiturates

Abstract

Retention volumes for a group of sulfonamides obtained on 3 different HPLC [high pressure liquid chromatography] columns were correlated with log P, pKa and biological activity. These data were compared with previously published RM values obtained from 5 different TLC systems. Previously published chromatographic data of barbiturates from 5 different HPLC systems were also correlated with log P, pKa and biological activity. Depending on the chromatographic system, good correlations can be obtained with log P or with biological activity, but not necessarily both, using the same chromatographic data.