'Anaphase' and cytokinesis in the absence of chromosomes
- 1 August 1996
- journal article
- Published by Springer Nature in Nature
- Vol. 382 (6590), 466-468
- https://doi.org/10.1038/382466a0
Abstract
Anaphase and cytokinesis are key processes in the segregation of replicated chromosomes to the daughter cells: in anaphase, chromosomes move apart; in cytokinesis, a cleavage furrow forms midway between the separated chromosomes. Some evidence suggests that chromosomes may be involved both in controlling the timing of anaphase onset and in dictating the position of the cleavage furrow. Other evidence indicates that the controlling mechanisms are intrinsic to the spindle and the cell. Here we test these possibilities in grasshopper spermatocytes by observing spindles and cells after removal of chromosomes. We found that both anaphase and cytokinesis occur independently of chromosomes: stage-specific changes occur at an appropriate time and in the correct way, despite the absence of chromosomes. This finding is particularly noteworthy because chromosomes have an important impact on spindle microtubule assembly and the timing of anaphase onset in these cells.Keywords
This publication has 13 references indexed in Scilit:
- Chromosomes initiate spindle assembly upon experimental dissolution of the nuclear envelope in grasshopper spermatocytes.The Journal of cell biology, 1995
- The impact of chromosomes and centrosomes on spindle assembly as observed in living cells.The Journal of cell biology, 1995
- Mitotic forces control a cell-cycle checkpointNature, 1995
- Does degradation lead to segregation?Current Biology, 1993
- Anaphase is initiated by proteolysis rather than by the inactivation of maturation-promoting factorCell, 1993
- Creative blocks: cell-cycle checkpoints and feedback controlsNature, 1992
- Role of the Centromere/Kinetochore in Cell Cycle ControlCold Spring Harbor Symposia on Quantitative Biology, 1991
- Structural and Mechanical Control of Mitotic ProgressionCold Spring Harbor Symposia on Quantitative Biology, 1991
- The reproduction of centrosomes: nuclear versus cytoplasmic controls.The Journal of cell biology, 1986
- Establishment of the Mechanism of Cytokinesis in Animal CellsInternational Review of Cytology, 1986