ANDROGEN BINDING IN CYTOSOLS AND NUCLEI OF HUMAN BENIGN HYPERPLASTIC PROSTATIC TISSUE

Abstract

Androgen binding sites have been detected in cytosols and nuclear extracts from human benign hyperplastic prostatic (BPH) tissue with exchange assays using [³H]methyltrienolone and [³H]5α-dihydrotestosterone respectively. The concentrations of binding sites and the equilibrium dissociation constants for the [³H]steroid–receptor interactions have been determined and the specificity of the binding has been examined. The observed properties of the binding sites are consistent with those characteristic of androgen receptors. The binding sites are present in nuclear extracts from all BPH tissue samples examined to date. The amount of binding detected in the nuclear fraction is higher than that found in the cytosol.