Separation of Progonadotropic and Antigonadotropic Activities in Ovine and Equine HCG Antisera

Abstract

The antigonadotropic factor (antibodies) was separated from the progonadotropic factors (probodies) in ovine and equine HCG antisera and in a gamma globulin fraction of equine HCG antiserum by passing the sera, or a serum salt fractionation product through a column in which HCG had been covalently coupled to activated Sepharose. The probodies passed through the column whereas the HCG antibodies were bound to the HCG on the column.