Possible Detection of HLA‐DR Alloantigenic Specificities in Man with Unabsorbed Rabbit Antisera

Abstract

Thirty‐six rabbits were immunized with precipitin lines formed between detergent solubilized membrane fractions from HLA‐D‐homozygous cells and a rabbit antiserum to human B cells (anti‐p 23/30). Thirty‐one of the rabbits produced B‐cell specific cytotoxic antibodies and twelve of the antibodies were also precipitating in gel diffusion. In cytotoxicity tests six of the antisera showed clear preferential reactivity with the immunizing HLA‐D (DR) antigen. Thus immunizazation of rabbits might prove valuable for the production of HLA‐DR typing reagents.