Mutation of the Zinc-Binding Metalloprotease Motif AffectsBacteroides fragilisToxin Activity but Does Not Affect Propeptide Processing
Open Access
- 1 August 2005
- journal article
- Published by American Society for Microbiology in Infection and Immunity
- Vol. 73 (8), 5273-5277
- https://doi.org/10.1128/iai.73.8.5273-5277.2005
Abstract
To evaluate the role of the zinc-binding metalloprotease in Bacteroides fragilis toxin (BFT) processing and activity, the zinc-binding consensus sequences (H348, E349, H352, G355, H358, and M366) were mutated by site-directed-mutagenesis. Our results indicated that single point mutations in the zinc-binding metalloprotease motif do not affect BFT processing but do reduce or eliminate BFT biologic activity in vitro.Keywords
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