Influence of organic anions on the liberation of penicillinase from Staphylococcus aureus

Abstract

A further fraction of cell-bound exopenicillinase, in addition to that bound ionically to the surface of the cell wall, has been identified in S. aureus. Treatment of cells with low concentrations (0.01[image]) of organic anions liberates part of this exopenicillinase, such liberation being dependent on both time and temperature, but independent of an external supply of energy. Tricarboxylic acids tend to be more effect than dicarboxylic or monocarboxylic, although there are large differences between ionic species even when tested at the same normalities.