Zur Chemie des Blutfarbstoffes. 4. Mitteilung. Über das Sulfhämoglobin (nebst Nachträgen zu den früheren Mitteilungen.)

Abstract

Crystals of the following hemoglobin compounds were produced: CO-Hb, NO-Hb, CN-Hb, Fluor-Hb, Sulf-Hb and Met-Hb. Their homogeneity was shown by 8pectrophotometrical methods. On the other hand it was proved by absorption spectra that a series of Hb-derivatiyes, described in the literature, is explained by superposition of absorption curves: to these so-called derivatives belong: sulfoxy-Hb (Borri 1903), CO-Sulf-Hb, Selen-Hb and CO-Se-Hb (Clarke and Hurtley 1907), Kat-Hb (van Klaveren), Nitrit-Hb, NO-Met-Hb (Anson and Mirsky 1925), AsH3-Hb. By cataphoretic experiments it is further shown that Hb is no salt-like combination of globin and the prosthetic group (Steudel and Peiser 1924); for both components move to the anode at pn 6.8, although the iso-electric point of globin has been found at pH 8.1 (Osato 1922). Concerning the work of Baudisch and Welo (1925) about the importance of magnetism on the catalytic faculty of iron, it is proved that the benzidine-reaction is called forth not only by paramagnetic Hb-derivatives but also by diamagnetic ones.