CHARACTERIZATION OF RECEPTORS MEDIATING 5‐HYDROXYTRYPTAMINE‐ AND CATECHOLAMINE‐INDUCED PLATELET AGGREGATION, ASSESSED BY THE ACTIONS OF α‐ AND β‐BLOCKERS, BUTYROPHENONES, 5‐HT ANTAGONISTS AND CHLORPROMAZINE

Abstract

1 Blood platelets from normal human volunteers were isolated and aggregated in vitro with 5-hydroxytryptamine (5-HT), noradrenaline (NA), dopamine and N-dimethyldopamine (DMDA). 2 The effects of 5-HT antagonists, α- and β-adrenoceptor blocking agents, butyrophenones and chlorpromazine upon aggregation induced by the four amines were investigated. 3 Only the 5-HT antagonists, chlorpromazine and spiroperidol were potent inhibitors of 5-HT-induced aggregation, and only phentolamine was a potent inhibitor of the catecholamine-induced aggregation. 4 Evidence was obtained for two populations of receptors, one for 5-HT and one for the three catecholamines.