Use of azide and hydrogen peroxide as an inhibitor for endogenous peroxidase in the immunoperoxidase method.
- 1 December 1987
- journal article
- research article
- Published by SAGE Publications in Journal of Histochemistry & Cytochemistry
- Vol. 35 (12), 1457-1460
- https://doi.org/10.1177/35.12.2824601
Abstract
We describe a simple and effective method for inhibition of endogenous peroxidase activity in the immunoperoxidase technique. Specimens are pre-treated with a mixture of azide and hydrogen peroxide, which is then followed by an indirect immunoperoxidase procedure. Comparison studies showed no significant loss of antigenicity or morphological details by this pre-treatment. The method is most useful for evaluating cell-specific antigens on specimens that have abundant endogenous peroxidase activity, such as blood and bone marrow.This publication has 1 reference indexed in Scilit:
- ENZYME-LABELED ANTIBODIES: PREPARATION AND APPLICATION FOR THE LOCALIZATION OF ANTIGENSJournal of Histochemistry & Cytochemistry, 1966