Cloning and identification of a DNA fragment coding for the sup1 gene of Saccharomyces cerevisiae
- 1 August 1984
- journal article
- Published by Springer Nature in Current Genetics
- Vol. 8 (6), 467-470
- https://doi.org/10.1007/bf00433913
Abstract
A plasmid, pYsup1-1, containing a DNA fragment able to suppress the recessive mutant phenotype of the suppressor locus sup1 (allele sup1-ts36) of Saccharomyces cerevisiae was isolated from a bank of yeast chromosomal DNA cloned in cosmid p3030. The complementing gene was localized on a 2.6 kb DNA fragment by further subcloning. Evidence is presented that the cloned DNA segment codes for the sup1 structural gene (chromosome IIR).Keywords
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