Expression cloning of an ATP receptor from mouse neuroblastoma cells.
- 1 June 1993
- journal article
- research article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 90 (11), 5113-5117
- https://doi.org/10.1073/pnas.90.11.5113
Abstract
Extracellular ATP activates cell-surface metabotropic and ionotropic nucleotide (P2) receptors in vascular, neural, connective, and immune tissues. These P2 receptors mediate a wealth of physiological processes, including nitric oxide-dependent vasodilation of vascular smooth muscle and fast excitatory neurotransmission in sensory afferents. Although ATP is now recognized as a signaling molecule, the cellular and molecular mechanisms underlying its actions have been difficult to study due to the absence of selective P2 receptor antagonists and cloned receptor genes. Nonetheless, five mammalian P2 receptor subtypes have been tentatively assigned based solely on agonist specificity and signaling properties. Here we report the cloning of a mouse cDNA encoding a P2 receptor that shares striking homology with several G protein-coupled peptide receptors. When expressed in Xenopus laevis oocytes, the cloned receptor resembles a metabotropic P2U receptor; activation by either ATP or UTP elicits the mobilization of intracellular calcium. mRNA encoding the P2U purinergic receptor is found in neural and nonneural tissues.Keywords
This publication has 34 references indexed in Scilit:
- Noradrenaline-ATP co-transmission in the sympathetic nervous systemTrends in Pharmacological Sciences, 1991
- Cloning and expression of a complementary DNA encoding a bovine adrenal angiotensin II type-1 receptorNature, 1991
- Molecular cloning of a functional thrombin receptor reveals a novel proteolytic mechanism of receptor activationCell, 1991
- Effects of Extracellular ATP on Mononuclear PhagocytesAnnals of the New York Academy of Sciences, 1990
- ATP as a CotransmitteraAnnals of the New York Academy of Sciences, 1990
- The P-loop — a common motif in ATP- and GTP-binding proteinsTrends in Biochemical Sciences, 1990
- The use of Xenopus oocytes to probe synaptic communicationTrends in Neurosciences, 1988
- cDNA eloping of bovine substance-K receptor through oocyte expression systemNature, 1987
- Is there a basis for distinguishing two types of P2-purinoceptor?General Pharmacology: The Vascular System, 1985
- A Method for Isolation of Intact, Translationally Active Ribonucleic AcidDNA, 1983