Factors responsible for oscillations of membrane potential recorded with tight-seal-patch electrodes in mouse fibroblasts
- 1 October 1988
- journal article
- research article
- Published by Springer Nature in The Journal of Membrane Biology
- Vol. 105 (1), 23-32
- https://doi.org/10.1007/bf01871103
Abstract
In giant fibroblastic L cells, penetration of a conventional microelectrode brought about marked decreases in the membrane potential and input resistance measured with a patch electrode under tight-seal whole-cell configuration, and repeated hyperpolarizations were often observed upon penetration. Therefore, the question arose whether such leakage artifact is a causal factor for generation of the membrane potential oscillation even in giant L cells. During whole-cell recordings, however, regular potential oscillations were observed in the cells that had not been impaled with a conventional microelectrode, as far as the Ca2+ buffer was not strong in the pipette solution. Oscillatory changes in the intracellular potential were detected by extracellular recordings with a tight-seal patch electrode in the cell-attached configuration. Thus, the potential oscillation occurs even in the absence of penetration-induced leakage or without rupture of the patch membrane. Withdrawal of a micropipette from one cell was often found to induce marked cell damage and elicit oscillatory hyperpolarizations in a neighboring cell with a certain time lag. The longer the distance between the injured and recorded cells, the greater was the time lag. Application of the cell lysate on the cell surface also gave rise to oscillatory hyperpolarizations. After repeated applications of the lysate, the membrane became unresponsive (desensitized), suggesting the involvement of receptors for the lysate factor. The lysates of different cell species (mouse lymphoma L5178Y cells or human epithelial Intestine 407 cells) produced similar effects. The effective component was heat stable and distinct from ATP. Lysate-induced hyperpolarizations were inhibited by deprivation of extracellular Ca2+ and by application of a Ca2+ channel blocker (nifedipine) or a K+ channel blocker (quinine) in the same manner as spontaneous oscillatory hyperpolarizations. It is concluded that the mouse fibroblast exhibits membrane potential oscillations, when the cell was activated, presumably via receptor systems, by some diffusible factors released from damaged cells.This publication has 18 references indexed in Scilit:
- Membrane voltage, resistance, and channel switching in isolated mouse fibroblasts (L cells): a patch‐electrode analysis.The Journal of Physiology, 1985
- Oscillatory hyperpolarizations and resting membrane potentials of mouse fibroblast and macrophage cell lines.The Journal of Physiology, 1984
- Calcium channel and calcium pump involved in oscillatory hyperpolarizing responses of L‐strain mouse fibroblastsThe Journal of Physiology, 1982
- Membrane potential changes associated with pinocytosis of serum lipoproteins in L cellsExperimental Cell Research, 1981
- Improved patch-clamp techniques for high-resolution current recording from cells and cell-free membrane patchesPflügers Archiv - European Journal of Physiology, 1981
- Effects of cytochalasin B and local anesthetics on electrical and morphological properties in L cellsExperimental Cell Research, 1981
- Oscillatory Membrane Potential Changes in Cells of Mesenchymal Origin: The Role of an Intracellular Calcium Regulating SystemJournal of Experimental Biology, 1979
- Oscillations of membrane potential in L cellsThe Journal of Membrane Biology, 1977
- The membrane potential of Ehrlich ascites tumor cells microelectrode measurements and their critical evaluationThe Journal of Membrane Biology, 1971
- The fibroblast in wound healing.1967