Conjugative transfer of a shuttle plasmid for Escherichia coli to Mycobacterium smegmatis

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Abstract
The chimeric plasmid pMY10 containing the origin of replication of pAL5000, the origin of replication of pBR322, the origin of transfer of pRK2 and a kanamycin resistance gene was constructed and succesfully transferred by conjugation from Escherichia coli harbouring the helper plasmid pRK4.24 into Mycobacterium smegmatis . This is the first report of conjugative transfer of plasmid between E. coli and an acid fast organism.