Positive screening and transformation of ura5 mutants in the fungus Podospora anserina: characterization of the transformants
- 1 July 1986
- journal article
- research article
- Published by Springer Nature in Current Genetics
- Vol. 10 (11), 811-817
- https://doi.org/10.1007/bf00418527
Abstract
Summary To develop a transformation system in the filamentous fungus Podospora anserina we have selected ura5 mutants deficient in orotidylic acid pyrophosphorylase using a positive screening. These mutants could be transformed to prototrophy by an hybrid vector carrying the ura5 gene of this organism. The properties of the transformants have been analysed. In most cases integration of the transforming vector occurred outside the ura5 locus and frequently repeated tandem copies of the vector were found. Reversion of the transformants could also be selected and we found that it can occur by exact or only partial excision of the integrated vector.Keywords
This publication has 16 references indexed in Scilit:
- Transformation by integration in Podospora anserinaMolecular Genetics and Genomics, 1985
- Development of a high-frequency transforming vector for Aspergillus nidulansGene, 1985
- Cloning gene ura5 for the orotidylic acid pyrophosphorylase of the filamentous fungus Podospora anserina: transformation of protoplastsGene, 1984
- A positive selection for mutants lacking orotidine-5′-phosphate decarboxylase activity in yeast: 5-fluoro-orotic acid resistanceMolecular Genetics and Genomics, 1984
- Transformation by integration in Aspergillus nidulansGene, 1983
- Studies on transformation of Escherichia coli with plasmidsJournal of Molecular Biology, 1983
- [19] Use of integrative transformation of yeast in the cloning of mutant genes and large segments of contiguous chromosomal sequencesMethods in Enzymology, 1983
- Cloned ural locus of Schizosaccharomyces pombe propagates autonomously in this yeast assuming a polymeric form.Proceedings of the National Academy of Sciences, 1982
- Labeling deoxyribonucleic acid to high specific activity in vitro by nick translation with DNA polymerase IJournal of Molecular Biology, 1977
- Detection of specific sequences among DNA fragments separated by gel electrophoresisJournal of Molecular Biology, 1975