Ganglioside sialidase activity in bovine neuronal perikarya
- 1 April 1976
- journal article
- Published by Springer Nature in Neurochemical Research
- Vol. 1 (2), 171-180
- https://doi.org/10.1007/bf00966108
Abstract
Neuronal perikarya were isolated, using bulk preparative procedures, from bovine brains. Synaptosomes, neuronal perikarya, and brain homogenates had similar ganglioside patterns, with the synaptosomes containing at least four times more total ganglioside per mg protein than the neuronal perikarya and twice that of the homogenate. Synaptosomes had 26–33 nmol total sialic acid/mg protein, while the neurons had only 15–17 nmol. Determination of ganglioside sialidase activity showed that neuronal perikarya had very low levels (negligible), in comparison with synaptosomes or whole-brain homogenates. Trypsin treatment during the isolation procedure enhanced sialidase activity two-to threefold in the particulate fraction of the whole-brain homogenate. Determination of the distribution of sialidase activity in the fractions obtained during the isolation of the neuronal perikarya showed that the sialidase activity was associated with the myelin, broken-off dendritic processes, and glial-cell fractions that banded in the less dense sucrose.Keywords
This publication has 30 references indexed in Scilit:
- Influence of cation concentration on the sialidase activity of neuronal synaptic membranesBiochemical and Biophysical Research Communications, 1975
- Studies on plasma membranesThe Journal of Membrane Biology, 1973
- Sub-synaptosomal localization of brain particulate neuraminidaseBrain Research, 1972
- Synaptic plasma membranes from rat brain synaptosomes: Isolation and partial characterizationBiochimica et Biophysica Acta (BBA) - Biomembranes, 1971
- Human brain sialidaseBiochemistry, 1970
- Proteolytic Enzymes Initiating Cell Division and Escape from Contact Inhibition of GrowthNature, 1970
- Neuronal Soma and Whole Neuroglia of Rat Brain: A New Isolation TechniqueScience, 1970
- Distribution of protein-bound N-acetylneuraminic acid in subcellular particulate fractions prepared from rat whole brainBiochimica et Biophysica Acta (BBA) - General Subjects, 1969
- A simple and accurate micromethod for quantitative determination of ganglioside patternsLife Sciences, 1964
- Quantitive estimation of sialic acidsBiochimica et Biophysica Acta, 1957