Genes, enzymes and membrane proteins of the nitrate respiration system ofEscherichia coli
- 1 December 1972
- journal article
- Published by Springer Nature in The Journal of Membrane Biology
- Vol. 9 (1), 195-207
- https://doi.org/10.1007/bf01868052
Abstract
A method was devised to isolate mutants carrying deletions through several genetic loci (chlD + andchlA +) which are involved in the membrane-bound nitrate respiratory complex ofEscherichia coli. Specific λ transducing phages were used to reintroduce these genes. Comparisons of membrane fractions from these transduced strains showed five membrane proteins that are necessary for the formation of an active nitrate respiration system. Two particular bacterial genes (chlD + andchlA +) were shown to control these five membrane proteins.Three of the proteins specified bychlA +, appear to be constitutively controlled and always present in the membrane ofE. coli irrespective of growth conditions, while the other two proteins, specified bychlD +, appear to be induced byanaerobic growth in the presence of nitrate.Keywords
This publication has 29 references indexed in Scilit:
- Etude des mutants chlorate-résistants chez Escherichia Coli K12 III. Mise en évidence et étude de l'activité chlorate-réductase c des mutants chl C−Biochimica et Biophysica Acta (BBA) - General Subjects, 1971
- Isolation of plaque forming λ phages which carry bacterial genes involved in the functional organization of the cell membraneBiochemical and Biophysical Research Communications, 1971
- Demonstration of missing membrane proteins in deletion mutants of E. coli K12Biochemical and Biophysical Research Communications, 1970
- Mapping of the gene Chl-B controlling membran bound nitrate reductase and formic hydrogen-lyase activities in Escherichia coli K 12Biochemical and Biophysical Research Communications, 1970
- Alteration of membrane proteins in a chlorate-resistant mutant of Escherichia coliBiochemical and Biophysical Research Communications, 1969
- Genetic mapping of the chl C gene of the nitrate reductase a system in Escherichia coli K12Biochemical and Biophysical Research Communications, 1969
- Colicins and Related BacteriocinsAnnual Review of Microbiology, 1967
- Alteration of respiratory particles by mutation in Escherichia coli K 12Biochemical and Biophysical Research Communications, 1967
- Regulation of metabolism in facultative bacteriaII. Effects of aerobiosis, anaerbiosis and nutrition on the formation of Krebs cycle enzymes in escherchia coliBiochimica et Biophysica Acta (BBA) - General Subjects, 1966
- Regulation of metabolism in facultative bacteria1. Structural and functional changes in Escherichia coli associated with shifts between the aerobic and anaerobic statesBiochimica et Biophysica Acta (BBA) - General Subjects, 1966