The Porcine Ovarian Follicle. IV. Mucopolysaccharides at Different Stages of Development1

Abstract

Concentrations of mucopolysaccharides (MPS) were measured in porcine follicular fluid by a high pressure liquid chromatographic (HPLC) procedure. Three peaks of material were apparent in follicular fluid of small (1-2 mm), medium (3-5 mm) and large (6-12 mm) follicles. Two peaks have been identified as chondroitin-like material and heparan sulfate. The third peak of small molecular size has not been characterized. Concentrations of chondroitin-like material decreased 8-fold with follicular maturation, while concentrations of heparan sulfate decreased 2-fold. Extracts prepared from granulosa cells aspirated with the follicular fluid also contain MPS and they were qualitatively similar to those found in follicular fluid except for the absence of Peak III material. The degree of sulfation of the chondroitins increased with follicular maturation. Gonadotropins and steroids may regulate synthesis and secretion of MPS by granulosa cells since production of MPS varies during maturation of the follicle. The physiological role of MPS in follicular and oocyte maturation, ovulation and atresia remains to be determined.