Initial studies on the application of high-performance liquid chromatography to determine organocopper speciation in soil-pore waters

Abstract

The use of reversed-phase high-performance liquid chromatographic techniques with molecular- and atomic-spectroscopic detectors to determine organocopper complexes in soil-pore wate is described. Polar dissolved organic compounds and associated copper complexes are separated using either a single Hypersil ODS column or two Hypersil ODS columns and a Hamilton PRP 1 column in series. Quantification was achieved using ultraviolet detectors for the organic molecular species and graphite furnace atomic-absorption spectrometry for the copper.